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KMID : 0545120060160091472
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Journal of Microbiology and Biotechnology
2006 Volume.16 No. 9 p.1472 ~ p.1476
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Gene Disruption Using In Vivo and In Vitro Methylation in Streptomyces griseus
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Maeng Jin-Soo
Bae Kyung-Sook
Kwak Jang-Yul
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Abstract
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Previous study demonstrated that the restriction barrier of Streptomyces griseus is almost completely bypassed by the Streptomyces-E. coli shuttle vectors passed through the E. coli GM161 strain and methylated with AluI and HpaII methyltransferases. The same DNA methylation of the genomic DNA fragments cloned the nonreplicative vectors generated integrative transformation and gene disruption of their chromosomal counterparts at high efficiencies in S. griseus. This result indicated that the efficiency of gene disruption depends on the efficient transfer of the incoming DNA into bacterial hosts.
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KEYWORD
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homologous recombination, Transformation, methylation, gene disruption, Streptomyces griseus
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